4CCS
The structure of CbiX, the terminal Enzyme for Biosynthesis of Siroheme in Denitrifying Bacteria
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 193 |
Detector technology | PIXEL |
Collection date | 2011-09-10 |
Detector | DECTRIS PILATUS 2M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 130.450, 130.450, 56.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.220 - 1.900 |
R-factor | 0.196 |
Rwork | 0.194 |
R-free | 0.23740 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.220 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.080 | 0.750 |
Number of reflections | 23026 | |
<I/σ(I)> | 24.9 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.2 | 14.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2M IMIDAZOLE MALATE PH 5.5, 24% PEG 600 | |||
1 | 0.2M IMIDAZOLE MALATE PH 5.5, 24% PEG 600 |