4CCF
Structure of Respiratory Syncytial Virus F protein head domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.380, 133.501, 116.420 |
| Unit cell angles | 90.00, 93.32, 90.00 |
Refinement procedure
| Resolution | 43.070 - 2.650 |
| R-factor | 0.21629 |
| Rwork | 0.215 |
| R-free | 0.24718 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rrr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.447 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.100 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 53253 | |
| <I/σ(I)> | 6.2 | 1.5 |
| Completeness [%] | 88.0 | 85.4 |
| Redundancy | 1.7 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG 8000, 200 MM AMMONIUM SULFATE, 200 MM SODIUM CACODYLATE PH 6.5 |
