4CC1

Notch ligand, Jagged-1, contains an N-terminal C2 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-24
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1
Unit cell lengths	55.950, 60.560, 63.030
Unit cell angles	92.89, 104.95, 105.92

Refinement procedure

Resolution	60.380 - 2.840
R-factor	0.1991
Rwork	0.196
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vj2
RMSD bond length	0.010
RMSD bond angle	1.230
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall
Low resolution limit [Å]	60.380
High resolution limit [Å]	2.840
Rmerge	0.160
Number of reflections	17793
<I/σ(I)>	11.3
Completeness [%]	98.2
Redundancy	2.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1