4CBP
Crystal structure of neural ectodermal development factor IMP-L2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.856, 99.732, 56.280 |
| Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
| Resolution | 29.542 - 1.600 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.18830 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.086 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.250 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.050 | 0.420 |
| Number of reflections | 70546 | |
| <I/σ(I)> | 13.4 | 2.3 |
| Completeness [%] | 95.7 | 71.9 |
| Redundancy | 3.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | HANGING-DROP EXPERIMENTS BY MIXING 1 UL PROTEIN (5.5. MG/ML IN 10 MM HEPES PH 7.4, 20 MM NACL) AND 1 UL RESERVOIR SOLUTION (17% PEG-6000, 0.1 M TRIS HYDROCHLORIDE, PH 7.0) |
