4CBI
Pestivirus NS3 helicase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.633, 96.303, 97.611 |
| Unit cell angles | 91.23, 110.86, 107.62 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.830 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 40172 | |
| <I/σ(I)> | 7.2 | 1.7 |
| Completeness [%] | 89.8 | 68.4 |
| Redundancy | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MES, 0.6 M NACL, 4% PEG, 1 MM AMPPCP, pH 6.5 |
