4CAI
Structure of inner DysF domain of human dysferlin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Collection date | 2012-09-15 |
| Detector | PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.894, 77.473, 74.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.505 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cah |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.040 | 0.470 |
| Number of reflections | 24062 | |
| <I/σ(I)> | 12.4 | 2.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.04M SODIUM DIHYDROGEN PHOSPHATE, 16% PEG 8000, 20% GLYCEROL, 0.2M NABR, pH 7.5 |
