4CAG
Bacillus licheniformis Rhamnogalacturonan Lyase PL11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 95.780, 95.780, 155.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.731 - 2.498 |
| R-factor | 0.1622 |
| Rwork | 0.159 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8r |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.532 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.140 | 0.600 |
| Number of reflections | 47873 | |
| <I/σ(I)> | 10.5 | 2.7 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15-20% PEG3350, 150 MM D,L-MALIC ACID PH 7.0 |
