4C9Y
Structural Basis for the microtubule binding of the human kinetochore Ska complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-07-27 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 39.006, 161.578, 104.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.868 - 2.010 |
R-factor | 0.1994 |
Rwork | 0.194 |
R-free | 0.24620 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.094 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.900 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.460 |
Number of reflections | 22542 | |
<I/σ(I)> | 15.2 | 3.8 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1M IMIDAZOLE-MES BUFFER, 0.09 M NPS MIX AND 30% EDO_P8K, pH 7.5 |