4C9I
Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 1E protein (Form B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.734, 82.459, 224.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 3.100 |
| R-factor | 0.21628 |
| Rwork | 0.214 |
| R-free | 0.26295 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c9c |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.518 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 29.890 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.080 | |
| Number of reflections | 27085 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 96.5 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRI-SODIUM CITRATE DIHYDRATE, 0.1M TRIS HYDROCHLORIDE PH 8.5 AND 5% PEG 400 |
