4C98

Thermus thermophilus Cas6 (TTHB231)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-20
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	68.540, 68.540, 133.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.465 - 2.001
R-factor	0.2064
Rwork	0.205
R-free	0.22100
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.007
RMSD bond angle	1.191
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.070	0.590
Number of reflections	22224
<I/σ(I)>	22.4	3.6
Completeness [%]	99.8	96.8
Redundancy	9.3	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1M MES (PH 6.0), 0.2M ZINC ACETATE, 9% (W/V) PEG 6000