4C97
Cas6 (TTHA0078) H37A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-04-04 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.930, 77.450, 88.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.366 - 1.700 |
R-factor | 0.169 |
Rwork | 0.168 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4c8y |
RMSD bond length | 0.010 |
RMSD bond angle | 1.349 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.400 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.080 | 0.730 |
Number of reflections | 56542 | |
<I/σ(I)> | 13.4 | 2 |
Completeness [%] | 98.3 | 99.7 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 0.1M BIS-TRIS PROPANE (PH 6.5), 16% PEG 335, 0.2 M SODIUM SULFATE |