4C97

Cas6 (TTHA0078) H37A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-04
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	73.930, 77.450, 88.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.366 - 1.700
R-factor	0.169
Rwork	0.168
R-free	0.19120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c8y
RMSD bond length	0.010
RMSD bond angle	1.349
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.400	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.080	0.730
Number of reflections	56542
<I/σ(I)>	13.4	2
Completeness [%]	98.3	99.7
Redundancy	4.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M BIS-TRIS PROPANE (PH 6.5), 16% PEG 335, 0.2 M SODIUM SULFATE