4C95
Crystal structure of the carboxy-terminal domain of yeast Ctf4 bound to Sld5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 88.944, 99.705, 218.577 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.919 - 2.694 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.21410 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.629 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.070 | 2.770 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.160 | 1.500 |
| Number of reflections | 54280 | |
| <I/σ(I)> | 10.3 | 1.4 |
| Completeness [%] | 99.2 | 90.5 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M TRI-SODIUM CITRATE PH 6.2, 7-9% PEG 8000 |
