4C94
Crystal Structure of the Strawberry Pathogenesis-Related 10 (PR-10) Fra a 3 protein in complex with Catechin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-07 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 137.899, 206.594, 174.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 3.000 |
| R-factor | 0.17861 |
| Rwork | 0.178 |
| R-free | 0.19933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c9c |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.573 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.030 | |
| Number of reflections | 49904 | |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 99.5 | |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.25 M SODIUM MALONATE, PH 7.0 |
