4C90
Evidence that GH115 alpha-glucuronidase activity is dependent on conformational flexibility
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.390, 131.680, 199.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.600 - 2.650 |
| R-factor | 0.20641 |
| Rwork | 0.203 |
| R-free | 0.26591 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.355 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCDE |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.520 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 219395 | |
| <I/σ(I)> | 8.5 | 2.4 |
| Completeness [%] | 97.5 | 94.5 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 19% PEG3350, 0.2M SODIUM CITRATE PH 5.5 |
