4C8Z
Cas6 (TTHA0078) product complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-14 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.720, 83.080, 72.380 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 54.034 - 2.503 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.21710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c8y |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.520 |
| Number of reflections | 17611 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M SODIUM SULFATE, 20% (W/V) PEG 3350, pH 7.5 |
