4C8S
Crystal structure of the C-terminal region of yeast Ctf4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-07-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 89.057, 100.251, 219.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.868 - 3.000 |
| R-factor | 0.1693 |
| Rwork | 0.167 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c8h |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.649 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.170 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.190 | 0.980 |
| Number of reflections | 40196 | |
| <I/σ(I)> | 12.6 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M TRI-SODIUM CITRATE PH 6.2, 7-9% PEG 8000 |
