4C8N
Binary complex of the large fragment of DNA polymerase I from Thermus Aquaticus with the aritificial base pair dNaM-d5SICS at the postinsertion site (sequence context 3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 287 |
| Detector technology | PIXEL |
| Collection date | 2012-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 65.617, 101.096, 204.316 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.069 - 1.880 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.23160 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.062 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 1.990 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.090 | |
| Number of reflections | 55373 | |
| <I/σ(I)> | 9.1 | 0.8 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 6.5 | 5.7 |
| CC(1/2) | 0.998 | 0.476 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.2M LITHIUM SULFATE, 2% W/V PEG 1000, 50MM HEPES PH 7.5 |
