4C88
Esterase LpEst1 from Lactobacillus plantarum: native structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 4 |
| Unit cell lengths | 168.340, 168.340, 184.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.678 - 2.650 |
| R-factor | 0.1384 |
| Rwork | 0.137 |
| R-free | 0.17490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.960 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.160 | 0.510 |
| Number of reflections | 74256 | |
| <I/σ(I)> | 12.1 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.3 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.8 M SODIUM ACETATE TRIHYDRATE PH 7.0 AND 35%(V/V) TACSIMATE PH 7.0 |
