4C7X
Thiamine Pyrophosphate Bound Transketolase from Lactobacillus salivarius at 2.2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.990, 74.990, 192.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.690 - 2.290 |
| R-factor | 0.15775 |
| Rwork | 0.154 |
| R-free | 0.23565 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c7v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.857 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.870 | 2.350 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.120 | 0.680 |
| Number of reflections | 29191 | |
| <I/σ(I)> | 15 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 15% W/V PEG3350, 0.1 M SODIUM ACETATE, 0.1 M BIS-TRIS PROPANE PH 7.5 |
