4C7V
Apo Transketolase from Lactobacillus salivarius at 2.2A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-09 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 76.150, 76.150, 194.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.140 - 2.200 |
| R-factor | 0.16524 |
| Rwork | 0.162 |
| R-free | 0.21917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.780 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.140 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 33992 | |
| <I/σ(I)> | 17.8 | 2.8 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 6.6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 % W/V PEG3350, 0.2 M SODIUM CHLORIDE |
