4C7N
Crystal Structure of the synthetic peptide iM10 in complex with the coiled-coil region of MITF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 23.701, 33.265, 61.125 |
| Unit cell angles | 90.00, 94.65, 90.00 |
Refinement procedure
| Resolution | 60.920 - 2.100 |
| R-factor | 0.22872 |
| Rwork | 0.228 |
| R-free | 0.25312 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.920 | 2.180 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 7041 | |
| <I/σ(I)> | 15.6 | 6.6 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 12.6 | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 3.5 M NAFORMATE PH 7.0 |
