4C76
Crystal Structure of the FMN-reductase Msue from Pseudomonas putida KT2440.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9798, 0.9762, 0.9800 |
| Spacegroup name | P 64 |
| Unit cell lengths | 124.730, 124.730, 45.340 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.500 - 1.960 |
| R-factor | 0.14751 |
| Rwork | 0.145 |
| R-free | 0.19252 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.408 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.120 | 0.620 |
| Number of reflections | 29204 | |
| <I/σ(I)> | 15.5 | 3.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 9.4 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1M TRIS-HCL PH 8.5, 25% W/V PEG 1K |
