4C74
Phenylacetone monooxygenase: Reduced enzyme in complex with APADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14 |
| Synchrotron site | ESRF |
| Beamline | ID14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.619, 107.619, 106.464 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.220 - 1.970 |
| R-factor | 0.23681 |
| Rwork | 0.235 |
| R-free | 0.27841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yls |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.105 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.080 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.160 | 1.000 |
| Number of reflections | 50703 | |
| <I/σ(I)> | 7.7 | 2.8 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 10.1 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | pH 6.5 |
