4C6O
Crystal structure of the dihydroorotase domain of human CAD C1613S mutant in apo-form at pH 6.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.120, 159.420, 61.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.927 - 1.650 |
| R-factor | 0.1168 |
| Rwork | 0.115 |
| R-free | 0.15710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c6c |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 1.690 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.080 | 0.610 |
| Number of reflections | 47108 | |
| <I/σ(I)> | 19.9 | 2.9 |
| Completeness [%] | 96.3 | 55.7 |
| Redundancy | 10.1 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | pH 6.0 |
