4C5Z
Crystal structure of A. niger ochratoxinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-05-07 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 183.940, 183.940, 79.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.280 - 2.500 |
| R-factor | 0.17704 |
| Rwork | 0.175 |
| R-free | 0.20890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3BE7 3feq 2R8C 3mtw 2qs8 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.441 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.180 | 0.610 |
| Number of reflections | 46056 | |
| <I/σ(I)> | 9.4 | 3.3 |
| Completeness [%] | 96.5 | 97.6 |
| Redundancy | 9.6 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 % (W/V) PEG 3000, 0.1 M TRIS PH 8.5, 0.2 M TRI-POTASSIUM CITRATE |
