4C41
Corticosteroid-binding globulin with engineered disulphide bridge between residues 100 and 236
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.130, 73.390, 126.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.977 - 1.800 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vdy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.803 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1702)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.980 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.160 | 0.820 |
| Number of reflections | 31617 | |
| <I/σ(I)> | 8.47 | 2.71 |
| Completeness [%] | 84.7 | 97.3 |
| Redundancy | 8.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.3 | 15% PEG3350, 0.2M NACL, 50MM MES, PH 5.3 |
