4C3I
Structure of 14-subunit RNA polymerase I at 3.0 A resolution, crystal form C2-100
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-18 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 400.530, 140.220, 122.890 |
| Unit cell angles | 90.00, 100.14, 90.00 |
Refinement procedure
| Resolution | 84.000 - 3.000 |
| R-factor | 0.2001 |
| Rwork | 0.199 |
| R-free | 0.23070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.000 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | |
| Number of reflections | 132738 | |
| <I/σ(I)> | 12.33 | 0.83 |
| Completeness [%] | 99.2 | 98.3 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10% MPD, 0.1M TRIS HCL PH 7.4 |
