4C2C
Crystal structure of the protease CtpB in an active state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 118.785, 118.785, 72.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1953 |
| Rwork | 0.195 |
| R-free | 0.21580 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.670 |
| Number of reflections | 46788 | |
| <I/σ(I)> | 36.5 | 5.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20.5 | 20.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.1 M NA-MALONATE PH 7.8 |
