4C24
L-fuculose 1-phosphate aldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 |
| Unit cell lengths | 112.970, 112.970, 40.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.720 - 1.500 |
| R-factor | 0.14062 |
| Rwork | 0.140 |
| R-free | 0.16066 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fua |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.351 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.480 |
| Number of reflections | 41479 | |
| <I/σ(I)> | 20.5 | 9.2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | SPFCSA AT A CONCENTRATION OF 14 MG PER ML, BUFFERED IN 20 MM TRIS-HCL (PH 8.0) AND 5 MM DITHIOTHREITOL (DTT) WAS CRYSTALLIZED BY MIXING EQUAL VOLUMES WITH A SOLUTION OF 4% (V PER V) GLYCEROL, 1.50 M AMMONIUM SULPHATE, 100 MM TRIS-HCL (PH 8.5) AND 8 MM ZINC CHLORIDE |
