4C23
L-fuculose kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 |
| Unit cell lengths | 60.624, 60.624, 280.013 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.980 - 2.000 |
| R-factor | 0.16863 |
| Rwork | 0.166 |
| R-free | 0.21597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cgk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.633 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 67536 | |
| <I/σ(I)> | 20.5 | 9.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | SPFCSK AT A CONCENTRATION OF 24 MG ML-1, BUFFERED IN 20 MM TRIS-HCL (PH 7.2), 150 MM NACL, 1 MM MANGANESE CHLORIDE, AND 1 MM DTT WAS CRYSTALLIZED BY MIXING EQUAL VOLUMES WITH A SOLUTION CONSISTING OF 4% (V PER V) ETHYLENE-GLYCOL, 100 MM 4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID (PH 7.5), AND 1.50 M AMMONIUM SULPHATE |
