4C22
L-Fucose Isomerase In Complex With Fuculose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-27 |
Detector | MARRESEARCH MX-300HE |
Spacegroup name | H 3 2 |
Unit cell lengths | 151.450, 151.450, 406.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.260 - 2.700 |
R-factor | 0.14675 |
Rwork | 0.144 |
R-free | 0.19836 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fui |
RMSD bond length | 0.011 |
RMSD bond angle | 1.563 |
Data reduction software | AutoProcess |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.110 | 0.450 |
Number of reflections | 49642 | |
<I/σ(I)> | 13.2 | 3.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.6 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | SPFCSI AT A CONCENTRATION OF 12 MG ML-1, BUFFERED IN 20 MM TRIS-HCL (PH 7.0), 150 MM NACL, AND 2 MM DTT WAS CRYSTALLIZED BY MIXING WITH EQUAL VOLUMES OF 100 MM TRIS-HCL (PH 8.0), 27% (W PER V) POLYETHYLENE GLYCOL 4,000, 50 MM NACL, AND 200 MM MAGNESIUM SULPHATE |