4C1O

Geobacillus thermoglucosidasius GH family 52 xylosidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-15
Detector	DECTRIS PILATUS 6M
Spacegroup name	C 2 2 21
Unit cell lengths	73.557, 105.143, 195.565
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.782 - 1.700
R-factor	0.142
Rwork	0.140
R-free	0.17300
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	195.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.530
Number of reflections	83431
<I/σ(I)>	18.6	2.7
Completeness [%]	99.9	100
Redundancy	5.1	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7		10MG/ML BETA-XYLOSIDASE IN 50MM TRIS-HCL PH8, 150MM NACL, MIXED EQUIVOLUME WITH 0.1M MES PH6, 0.4M AMMONIUM SULPHATE AND 25% PEG3350. CRYSTALS WERE GROWN BY THE HANGING DROP VAPOUR DIFFUSION METHOD., pH 7