4C0Z
The N-terminal domain of the Streptococcus pyogenes pilus tip adhesin Cpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-13 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 31 |
| Unit cell lengths | 132.220, 132.220, 136.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.500 - 2.000 |
| R-factor | 0.2026 |
| Rwork | 0.201 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.910 | 2.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.160 | |
| Number of reflections | 1069788 | |
| <I/σ(I)> | 4.94 | 0.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.8 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 16 % PEG3350, 0.3 M KH2PO4, pH 7.4 |
