4C0P
Unliganded Transportin 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 96.198, 104.019, 124.890 |
| Unit cell angles | 90.64, 97.88, 104.48 |
Refinement procedure
| Resolution | 38.170 - 2.950 |
| R-factor | 0.2373 |
| Rwork | 0.236 |
| R-free | 0.26580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2X19 2xwu |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.140 | 1.130 |
| Number of reflections | 96311 | |
| <I/σ(I)> | 8.1 | 1.4 |
| Completeness [%] | 98.6 | 98.7 |
| Redundancy | 3.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 20% (W/V) PEG3350, 0.2 M NABR, 10 MM DTT, 2 MM EDTA, 0.1 M BIS-TRIS PROPANE, PH8.2 |
