4C0L
Crystal structure of Drosophila Miro EF hand and cGTPase domains bound to one magnesium ion and Mg:GDP (MgGDP-MiroS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-07 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.510, 81.510, 154.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.825 - 3.000 |
| R-factor | 0.2166 |
| Rwork | 0.214 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INITIAL MODEL FROM PRIOR DATASET |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.523 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.200 | 3.240 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.580 |
| Number of reflections | 12487 | |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 5.0 MG/ML MIROS,1.9 M LISO4, 0.1 M BIS-TRIS PH 8.0, 5 MM EGTA; CRYSTALS WERE SOAKED IN 30 MM MGCL2, 20 MM GDP FOR 72H PRIOR TO DATA COLLECTION. |
