4C0G
Structure of the NOT-box domain of human CNOT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000, 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.300, 97.570, 141.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.058 - 2.400 |
| R-factor | 0.2226 |
| Rwork | 0.221 |
| R-free | 0.25660 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.615 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 28972 | |
| <I/σ(I)> | 18.1 | 3.2 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 5.9 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.8 | 100 MM HEPES PH=7.8, 13% PEG4000, 2% GLYCEROL, 12% ISOPROPANOL |
