4C0G

Structure of the NOT-box domain of human CNOT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-10-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000, 0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	52.300, 97.570, 141.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.058 - 2.400
R-factor	0.2226
Rwork	0.221
R-free	0.25660
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.002
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.460
High resolution limit [Å]	2.400	2.400
Number of reflections	28972
<I/σ(I)>	18.1	3.2
Completeness [%]	99.5	99.8
Redundancy	5.9	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8		100 MM HEPES PH=7.8, 13% PEG4000, 2% GLYCEROL, 12% ISOPROPANOL