4C0E
Structure of the NOT1 superfamily homology domain from Chaetomium thermophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.750, 127.220, 130.200 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 63.610 - 3.200 |
| R-factor | 0.2189 |
| Rwork | 0.217 |
| R-free | 0.25860 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.526 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.900 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.110 | 0.540 |
| Number of reflections | 41851 | |
| <I/σ(I)> | 11.3 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100MM MES PH=6.0, 180MM MGCL2, 5% PEG20000, 10MM PROLINE |
