4BZE

Crystal structure of galactose mutarotase GalM from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-09-24
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	82.340, 82.906, 123.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.450 - 2.000
R-factor	0.19747
Rwork	0.195
R-free	0.23983
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mwx
RMSD bond length	0.013
RMSD bond angle	1.477
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.400	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.970
Number of reflections	57834
<I/σ(I)>	16.6	2.8
Completeness [%]	100.0	100
Redundancy	13.2	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1