4BZA
Crystal structure of TamA POTRA domains 1-3 from E. coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-24 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.050, 83.050, 150.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.974 - 1.839 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.20150 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.464 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.950 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.050 | 1.730 |
| Number of reflections | 46484 | |
| <I/σ(I)> | 31.36 | 1.99 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 12.99 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HEPES PH 7.5, DI-NA TARTRATE, PEG 3350, NACL, GLYCEROL |
