4BYG
ATPase crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-06-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.120, 72.910, 329.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.984 - 2.850 |
| R-factor | 0.235 |
| Rwork | 0.232 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rfu |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 1.210 |
| Number of reflections | 23095 | |
| <I/σ(I)> | 17.4 | 2.2 |
| Completeness [%] | 89.0 | 37 |
| Redundancy | 6.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 6 % (W/V) PEG6K, 10 % (V/V) GLYCEROL, 140 MM NACL, 3 % V/V T-BUOH, 5 MM BME, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
