4BY3
Crystal structure of the dihydroorotase domain of human CAD in apo- form obtained recombinantly from E. coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2010-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.120, 159.320, 61.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.856 - 1.730 |
| R-factor | 0.1262 |
| Rwork | 0.126 |
| R-free | 0.13620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z00 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.770 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.090 | 0.590 |
| Number of reflections | 42366 | |
| <I/σ(I)> | 14.6 | 3.1 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 6.5 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
