4BXQ
Structure of the E1021V mutant of the TCP10 domain of Danio rerio CPAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-11-11 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.120, 36.480, 56.460 |
| Unit cell angles | 90.00, 117.47, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.900 |
| R-factor | 0.21212 |
| Rwork | 0.209 |
| R-free | 0.26682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bxp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 1.090 |
| Number of reflections | 15136 | |
| <I/σ(I)> | 13.8 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 80 MM TRIS PH 8.5, 160 MM MGCL2, 24% PEG-4000, 20% GLYCEROL |
