4BXC
Resolving the activation site of positive regulators in plant phosphoenolpyruvate carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 164.413, 121.232, 132.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.680 - 2.860 |
R-factor | 0.18478 |
Rwork | 0.184 |
R-free | 0.22067 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zge |
RMSD bond length | 0.013 |
RMSD bond angle | 1.619 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.900 | 2.990 |
High resolution limit [Å] | 2.840 | 2.840 |
Rmerge | 0.050 | 0.420 |
Number of reflections | 60347 | |
<I/σ(I)> | 12.4 | 1.9 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.6 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.6 | 0.1 M TRI-SODIUM CITRATE (PH 5.6/NAOH), 15 % (W/V) PEG4000 AND 0.2 M AMMONIUM SULFATE; SOAKED WITH 200 MM GLUCOSE 6-PHOSPHATE |