4BX6
trans-divalent streptavidin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-07 |
| Detector | MARRESEARCH MX-300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.040, 65.120, 81.590 |
| Unit cell angles | 90.00, 95.94, 90.00 |
Refinement procedure
| Resolution | 24.981 - 1.595 |
| R-factor | 0.1652 |
| Rwork | 0.164 |
| R-free | 0.18660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ry1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.980 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.640 |
| Number of reflections | 64074 | |
| <I/σ(I)> | 13.88 | 1.92 |
| Completeness [%] | 98.0 | 97.3 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 65% V/V 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M 2-(N-MORPHOLINO)ETHANESULFONIC ACID (MES) PH 6.0. CRYSTALS WERE OBTAINED BY THE SITTING-DROP VAPOR-DIFFUSION METHOD AT 291 K AND REACHED A MAXIMUM SIZE AFTER 10 DAYS AND WERE HARVESTED SOON AFTER |
