4BWV
Structure of Adenosine 5-prime-phosphosulfate Reductase apr-b from Physcomitrella Patens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-17 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.990, 82.420, 127.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.800 - 1.800 |
| R-factor | 0.16419 |
| Rwork | 0.163 |
| R-free | 0.19433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oq2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.477 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.530 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.460 |
| Number of reflections | 54939 | |
| <I/σ(I)> | 12.6 | 2.3 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | CRYSTALLIZED AT 20 DEGREES C BY HANGING-DROP VAPOUR DIFFUSION USING A PRECIPITANT CONSISTING OF 25% PEG 5000 MME IN 100 MM BIS-TRIS PH 7.5 |
