4BWJ
KlenTaq mutant in complex with DNA and ddCTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.143, 108.143, 89.810 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.827 - 1.550 |
| R-factor | 0.1605 |
| Rwork | 0.158 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rtv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.504 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.830 | 1.640 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 1.120 |
| Number of reflections | 88092 | |
| <I/σ(I)> | 15.56 | 1.64 |
| Completeness [%] | 99.5 | 98 |
| Redundancy | 8.57 | 8.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | HANGING DROP. 100MM TRIS PH 7.5, 200MM MG-FORMATE, 15% PEG 8K |
