4BWI
Structure of the phytochrome Cph2 from Synechocystis sp. PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.430, 88.460, 84.870 |
| Unit cell angles | 90.00, 109.85, 90.00 |
Refinement procedure
| Resolution | 29.632 - 2.600 |
| R-factor | 0.2026 |
| Rwork | 0.200 |
| R-free | 0.25300 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.622 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 | 0.050 |
| Number of reflections | 32838 | |
| <I/σ(I)> | 21 | 3.4 |
| Completeness [%] | 98.7 | 98.7 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | (20 MG/ML SYNCPH2(1-2) IN PR IN 10 MM HEPES, 100 MM NACL, PH 8.0); (0.1 M HEPES PH 7.0, 1.0 M NH4COOH, 0.1% (W/V) LYSINE, 0.16% (W/V) ARGININE, 0.05% (W/V) GLUTAMATE); STREAK SEEDING, HANGING DROP |
