4BW7
Calmodulin in complex with strontium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.340, 53.940, 59.370 |
| Unit cell angles | 88.51, 96.61, 93.96 |
Refinement procedure
| Resolution | 19.604 - 1.810 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1up5 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.656 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.050 | 0.260 |
| Number of reflections | 25640 | |
| <I/σ(I)> | 10.8 | 2.4 |
| Completeness [%] | 93.9 | 82.1 |
| Redundancy | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | pH 4 |
