4BW0
The molecular recognition of kink turn structure by the L7Ae class of proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2013-03-17 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 61.920, 61.920, 130.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.979 - 2.330 |
| R-factor | 0.1814 |
| Rwork | 0.180 |
| R-free | 0.20090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rlg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 11309 | |
| <I/σ(I)> | 20 | 2.9 |
| Completeness [%] | 98.0 | 92 |
| Redundancy | 6.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.3M AMMONIUM SULFATE, 0.1M HEPES-NA PH 7.0 |
