4BVM
The peripheral membrane protein P2 from human myelin at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 58.070, 58.070, 101.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.163 - 0.930 |
| R-factor | 0.104 |
| Rwork | 0.104 |
| R-free | 0.12140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wut |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.074 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.950 |
| High resolution limit [Å] | 0.930 | 0.930 |
| Rmerge | 0.040 | 0.840 |
| Number of reflections | 114629 | |
| <I/σ(I)> | 22.5 | 1.1 |
| Completeness [%] | 98.2 | 84.4 |
| Redundancy | 6.5 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | pH 5 |
